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(A-C) Colonization data of PMSS1 and either an (A-B) isometric one-day isolate (G or I in ) or an (C) altered LPS electrophoretic mobility one-day isolate (H in ) coinfected into 10 C57BL6/N mice each for 1 week. Points represent the recovered CFU per gram of stomach tissue from each mouse and lines connect output strains from the same mice. LOD: limit of detection. (A-C) Points with the same shape indicate data from the same biological replicate. Replicates 1 (open circle) and 3 (open triangle) were performed with cassette-marked Isolates G, I, or H in female mice, while Replicate 2 (open square) was performed with cassette-marked PMSS1 in male mice. (D-E) Amplicon <t>sequencing</t> of futB from the stomach homogenate of representative mice from (D) PMSS1/G or I or (E) PMSS1/H coinfections displayed as the percentage of sequencing reads harboring each repeat length of the C-terminal 21-bp repeat (rpt) motif. Input and expected outputs are based on WGS and CFU data.
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(A-C) Colonization data of PMSS1 and either an (A-B) isometric one-day isolate (G or I in ) or an (C) altered LPS electrophoretic mobility one-day isolate (H in ) coinfected into 10 C57BL6/N mice each for 1 week. Points represent the recovered CFU per gram of stomach tissue from each mouse and lines connect output strains from the same mice. LOD: limit of detection. (A-C) Points with the same shape indicate data from the same biological replicate. Replicates 1 (open circle) and 3 (open triangle) were performed with cassette-marked Isolates G, I, or H in female mice, while Replicate 2 (open square) was performed with cassette-marked PMSS1 in male mice. (D-E) Amplicon <t>sequencing</t> of futB from the stomach homogenate of representative mice from (D) PMSS1/G or I or (E) PMSS1/H coinfections displayed as the percentage of sequencing reads harboring each repeat length of the C-terminal 21-bp repeat (rpt) motif. Input and expected outputs are based on WGS and CFU data.
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(A-C) Colonization data of PMSS1 and either an (A-B) isometric one-day isolate (G or I in ) or an (C) altered LPS electrophoretic mobility one-day isolate (H in ) coinfected into 10 C57BL6/N mice each for 1 week. Points represent the recovered CFU per gram of stomach tissue from each mouse and lines connect output strains from the same mice. LOD: limit of detection. (A-C) Points with the same shape indicate data from the same biological replicate. Replicates 1 (open circle) and 3 (open triangle) were performed with cassette-marked Isolates G, I, or H in female mice, while Replicate 2 (open square) was performed with cassette-marked PMSS1 in male mice. (D-E) Amplicon sequencing of futB from the stomach homogenate of representative mice from (D) PMSS1/G or I or (E) PMSS1/H coinfections displayed as the percentage of sequencing reads harboring each repeat length of the C-terminal 21-bp repeat (rpt) motif. Input and expected outputs are based on WGS and CFU data.

Journal: bioRxiv

Article Title: Helicobacter pylori allelic variation in cell surface genes influences human exoproteome binding and stomach tissue adherence

doi: 10.64898/2026.03.06.710112

Figure Lengend Snippet: (A-C) Colonization data of PMSS1 and either an (A-B) isometric one-day isolate (G or I in ) or an (C) altered LPS electrophoretic mobility one-day isolate (H in ) coinfected into 10 C57BL6/N mice each for 1 week. Points represent the recovered CFU per gram of stomach tissue from each mouse and lines connect output strains from the same mice. LOD: limit of detection. (A-C) Points with the same shape indicate data from the same biological replicate. Replicates 1 (open circle) and 3 (open triangle) were performed with cassette-marked Isolates G, I, or H in female mice, while Replicate 2 (open square) was performed with cassette-marked PMSS1 in male mice. (D-E) Amplicon sequencing of futB from the stomach homogenate of representative mice from (D) PMSS1/G or I or (E) PMSS1/H coinfections displayed as the percentage of sequencing reads harboring each repeat length of the C-terminal 21-bp repeat (rpt) motif. Input and expected outputs are based on WGS and CFU data.

Article Snippet: Samples were quantified and quality checked by Tapestation (Agilent 4200), then submitted for standard bacterial genome sequencing through Plasmidsaurus.

Techniques: Amplification, Sequencing